hosting/serology/nrl-pub.nsf

















Specimen Requirements for Reference Testing


SPECIMEN COLLECTION AND TRANSPORTATION REQUIREMENTS


Analyte
Minimum Specimen Requirements
Anti-HCV
Anti-HTLV-I/II
Anti HIV-1/2
  • 1mL serum or plasma. Transport on cold packs to arrive at the NRL as soon as possible following collection but within 7 days. Refrigerate during transit.
HIV-1 p24 antigen
  • 1mL serum or plasma. Transport on cold packs to arrive at the NRL as soon as possible following collection but within 7 days. Refrigerate during transit.
  • Otherwise, freeze sample and transport on dry ice.
HIV & HTLV DNA
  • Dedicated 5 – 10mL whole blood collected into EDTA or ACD. Transport at 4 - 25oC to arrive at the NRL preferably within 5 days, but up to 28 days after collection*
  • If greater than 5 days, an appropriate specimen for antibody testing (see above) must be supplied except when arranged otherwise with NRL.
HCV RNA
  • 5mL whole blood collected into EDTA or ACD. Transport at 4 – 25oC to the NRL within 24 hours of collection; OR
  • 1.5 mL plasma separated from cells using aseptic techniques. Transport to the NRL at ambient temperature (15 - 25oC) within 24 hours of collection; OR
  • 1.5 mL plasma separated from cells using aseptic techniques then frozen. Transport to the NRL on dry ice if longer than 24 hours after collection.
Anti-HIV-1, anti-HCV
(Dried blood spot)
  • One drop of whole blood to completely fill a circle (1cm diameter), on Schleischer & Schuell 903 filter paper. Transport at ambient temperature (15 - 25oC) with desiccant.
Results to be used for investigational use only

* It is recommended that samples for DNA detection arrive as soon as possible following collection.

Packaging
Specimens that are sent by airfreight must comply with the following conditions:
    1. The sample should be packed and shipped in accordance with the IATA Dangerous Goods Regulations
    2. For specimens referred from outside Australia, a copy of the NRL import permit and customs declaration should be attached to the parcel. A copy of the import permit and customs declaration template will be faxed on request.
    3. The parcel should be addressed to:
blank table spaceA/Prof. Elizabeth M. Dax
Director
National Serology Reference Laboratory, Australia
4th Floor, Healy Building
41 Victoria Parade
FITZROY VIC 3065
AUSTRALIA
TEL: + 61-3-9418 1111

Packages should not arrive on the weekend without prior arrangement.